Datasheet Protocol

Human TLR3 ELISA Kit

Name: Human TLR3 ELISA Kit
Brand: Rockland Immunochemicals
Price: 639.0 USD
Availability: OutOfStock

Item No. KOA0547

Size: 1 Kit

Applications: ELISA

Reactivity: Human

Range: 156 pg/ml - 10000 pg/ml

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Specifications for Human TLR3 ELISA Kit

Product Details

Description: Human TLR3 AccuSignal ELISA Kit - KOA0547

Synonyms: CD_antigen=CD283, CD283, IIAE2, Toll like receptor 3

Detection Kit Type: ELISA Kit

Detection Range: 156 pg/ml - 10000 pg/ml

Target Details

Gene Name: TLR3 - View All TLR3 Products

Reactivity: Human

Immunogen: Expression system for standard: NSO; Immunogen sequence: K27-S711

Purity/Specificity: Natural and recombinant human TLR3. There is no detectable cross-reactivity with other relevant proteins.

Database Links:

O15455 - UniProtKB

NP_003256.1 - NCBI Protein

7098 - Gene ID

Application Details

Tested Applications: ELISA

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human TLR3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human TLR3 standard solution and each sample be measured in duplicate.

Formulation

Anticoagulant: Heparin Sodium

Concentration: 1 Kit

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiration: See kit insert for complete instructions.

Background

Toll-like receptor 3(TLR3) also known as CD283(cluster of differentiation 283) is a protein that in humans is encoded by the TLR3 gene. The TLR3 gene was mapped to chromosome 4q35 by fluorescence in situ hybridization. TLR3 specifically recognizes double-stranded RNA(dsRNA) and induces multiple intracellular events responsible for innate antiviral immunity against a number of viral infections.

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.