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Human Tissue factor - F3 ELISA Kit

KOA0564
1 Kit
ELISA
Human
15.6 pg/ml - 1000 pg/ml
$589.00 /Per Item
Made to order
Ships in approximately 21 days
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Product Details

Human Tissue Factor/F3 AccuSignal ELISA Kit - KOA0564
CD142, Coagulation factor III, F3, TF, TF_HUMAN, TFA, Thromboplastin, Tissue factor
ELISA Kit
15.6 pg/ml - 1000 pg/ml

Target Details

F3  - View All F3 Products
Human
Expression system for standard: NSO; Immunogen sequence: S33-E251
Natural and recombinant human Tissue factor. There is no detectable cross-reactivity with other relevant proteins.
P13726 - UniProtKB
NP_001171567.1 - NCBI Protein

Application Details

ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml human Tissue factor standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) cell lysates or urine to each empty well. It is recommended that each human Tissue factor standard solution and each sample be measured in duplicate.

Formulation

Heparin Sodium

Shipping & Handling

Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Tissue factor, also called platelet tissue factor, factor III, thrombokinase, or CD142 is a protein present in sub endothelial tissue and leukocytes necessary for the initiation of thrombin formation from the zymogene prothrombin. An incorrect synonym is thromboplastin. the F3 gene was mapped to 1pter-p21 by study of somatic cell hybrids with a species-specific sensitive chromogenic assay. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII.

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