Human TFF3 ELISA Kit
KOA0726
1 Kit
ELISA
Human
62.5 pg/ml - 4000 pg/ml
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Human TFF3 AccuSignal ELISA Kit - KOA0726
hITF, hP1.B, Intestinal trefoil factor, ITF, mITF, OTTMUSP00000021729, Polypeptide P1.B, TFF3, TFF3_HUMAN, TFI, TREFOIL, Trefoil factor (intestinal), Trefoil factor 3
ELISA Kit
62.5 pg/ml - 4000 pg/ml
Target Details
TFF3 - View All TFF3 Products
Human
Expression system for standard: E.coli; Immunogen sequence: E22-Y80
Natural and recombinant human TFF3. There is no detectable cross-reactivity with other relevant proteins.
Q07654 - UniProtKB
NP_003217.3 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml human TFF3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva or urine to each empty well. It is recommended that each human TFF3 standard solution and each sample be measured in duplicate.
Formulation
Heparin Sodium
1 kit
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. It is mapped to 21q22.3. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to antiapoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. This gene is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies.
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