Human PEDF - SerpinF1 ELISA Kit
KOA0537
1 Kit
ELISA
Human
156 pg/ml - 10,000 pg/ml
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Human PEDF/SerpinF1 AccuSignal ELISA Kit - KOA0537
Alpha 2 antiplasmin, Cell proliferation-inducing gene 35 protein, EPC1, OI12, OI6, PEDF, PEDF_HUMAN, PIG35, Pigment epithelium derived factor, Pigment epithelium-derived factor, Proliferation inducing protein 35, serine (or cysteine) proteinase inhibitor clade F (alpha-2 antiplasmin, pigment epithelium derived factor) member 1, SERPIN F1, Serpin peptidase inhibitor clade F member , |serpin peptidase inhibitor clade F (alpha-2 antiplasmin pigment epithelium derived factor) member 1, SERPINF1
ELISA Kit
156 pg/ml - 10,000 pg/ml
Target Details
SERPINF1 - View All SERPINF1 Products
Human
Expression system for standard: NSO; Immunogen sequence: M1-P418
Natural and recombinant human PEDF. There is no detectable cross-reactivity with other relevant proteins.
P36955 - UniProtKB
NP_002606.3 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 50ng/ml, 25ng/ml, 12.5ng/ml, 6.25ng/ml, 3.12ng/ml, 1.56ng/ml, 0.78ng/ml human PEDF standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human PEDF standard solution and each sample is measured in duplicate.
Formulation
Heparin Sodium
1 Kit
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Pigment epithelium-derived factor (PEDF) is also known as serpin F1 (SERPINF1). In humans, it is encoded by the SERPINF1 gene. PEDF has a variety of functions including antiangiogenic, antitumorigenic, and neurotrophic properties. It suppresses retinal neovascularization and endothelial cell proliferation. And Antiangiogenic function is also conferred by PEDF through inhibition of both VEGFR-1 and VEGFR-2. In addition, the antitumorigenic effects of PEDF are not only due to inhibition of supporting vasculature, but also due to effects on the cancer cells themselves. PEDF is shown to inhibit cancer cell proliferation and increase apoptosis via the FAS/FASL pathway. Expression of PEDF in the human retina is found at 7.4 weeks of gestation, suggesting it may play a role in retinal neuron differentiation.
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