Datasheet Protocol

Human PD-1 ELISA Kit

Name: Human PD-1 ELISA Kit
Brand: Rockland Immunochemicals
Price: 639.0 USD
Availability: OutOfStock

Item No. KOA0589

Size: 1 Kit

Applications: ELISA

Reactivity: Human

Range: 156 pg/ml - 10000 pg/ml

$639.00 /Per Item

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Specifications for Human PD-1 ELISA Kit

Product Details

Description: Human PD-1 AccuSignal ELISA Kit - KOA0589

Synonyms: CD279, CD279 antigen, hPD 1, hPD-1, PD 1, PD-1, PD1, PDCD 1, PDCD1, PDCD1_HUMAN, Programmed cell death 1, Programmed cell death protein 1, Protein PD 1, Protein PD-1, SLEB2

Detection Kit Type: ELISA Kit

Detection Range: 156 pg/ml - 10000 pg/ml

Target Details

Gene Name: PDCD1 - View All PDCD1 Products

Reactivity: Human

Immunogen: Expression system for standard: NSO; Immunogen sequence: L25-Q167

Purity/Specificity: Natural and recombinant human PD-1. There is no detectable cross-reactivity with other relevant proteins.

Database Links:

Q15116 - UniProtKB

NP_005009.2 - NCBI Protein

Application Details

Tested Applications: ELISA

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human PD-1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates to each empty well. It is recommended that each human PD-1 standard solution and each sample be measured in duplicate.

Formulation

Concentration: 1 Kit

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiration: See kit insert for complete instructions.

Background

Programmed cell death protein 1, also called PD-1 or PDCD1 is a protein that in humans is encoded by the PDCD1 gene. This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. By fluorescence in situ hybridization, the PDCD1 gene is mapped to 2q37.3. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.