Human P-Selectin ELISA Kit
KOA0301
1 Kit
ELISA
Human
62.5 pg/ml - 4000 pg/ml
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Human P-Selectin AccuSignal ELISA Kit - KOA0301
Antigen CD62, CD62, CD62 antigen like family member P, GMP140, Granule membrane protein 140, Granule membrane protein 140kDa, GRMP, LECAM3, Leukocyte endothelial cell adhesion molecule 3, Leukocyte-endothelial cell adhesion molecule 3, LYAM3_HUMAN, P selectin, P-selectin, PADGEM, Platelet activation dependent granule external membrane protein, Platelet activation dependent granule-external membrane protein, Platelet alpha granule membrane protein, PSEL, Selectin P
ELISA Kit
62.5 pg/ml - 4000 pg/ml
Target Details
SELP - View All SELP Products
Human
Expression system for standard: NSO; Immunogen sequence: W42-A771
Natural and recombinant human sP-Selectin. There is no detectable cross-reactivity with other relevant proteins.
P16109 - UniProtKB
NP_002996.2 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human P-Selectin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human P-Selectin standard solution and each sample be measured in duplicate.
Formulation
Heparin Sodium
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1(PSGL-1). The standard product used in this kit is recombinant human P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 730 amino acids sequence with the molecular mass of 80 KDa.
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