Human MPO ELISA Kit
KOA0511
1 Kit
ELISA
Human
312 pg/ml - 20000 pg/ml
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Human MPO AccuSignal ELISA Kit - KOA0511
84 kDa myeloperoxidase, 89 kDa myeloperoxidase, MPO, MPO Myeloperoxidase, Myeloperoxidase, Myeloperoxidase heavy chain, Myeloperoxidase light chain, PERM_HUMAN
ELISA Kit
312 pg/ml - 20000 pg/ml
Target Details
MPO - View All MPO Products
Human
Expression system for standard: NSO; Immunogen sequence: A49-S745
Natural and recombinant human MPO. There is no detectable cross-reactivity with other relevant proteins.
P05164 - UniProtKB
NP_000241.1 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml human MPO standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, cell lysate, tissue lysate, serum, plasma(heparin, EDTA), saliva or urine to each empty well. It is recommended that each human MPO standard solution and each sample be measured in duplicate.
Formulation
Heparin Sodium
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Myeloperoxidase(MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function. The standard product used in this kit is the product of gene recombination, consisting of 697(A49-S745) amino acids with the molecular mass of 80KDa.
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