Human MMP13 ELISA Kit
KOA0275
1 Kit
ELISA
Human
156 pg/ml - 10000 pg/ml
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Human MMP13 AccuSignal ELISA Kit - KOA0275
CLG 3, CLG3, Collagenase 3, Collagenase3, MANDP1, Matrix metalloproteinase 13, Matrix metalloproteinase-13, MMP 13, MMP-13, Mmp13, MMP13_HUMAN, UMRCASE
ELISA Kit
156 pg/ml - 10000 pg/ml
Target Details
MMP13 - View All MMP13 Products
Human
Expression system for standard: E.coli; Immunogen sequence: L20-C471
Natural and recombinant human total MMP13. There is no detectable cross-reactivity with other relevant proteins.
P45452 - UniProtKB
NP_002418.1 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human MMP13 standard solutions into the pre-coated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma( heparin) to each empty well. It is recommended that each human MMP13 standard solution and each sample be measured in duplicate.
Formulation
Heparin Sodium
1 Kit
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. The expression of MMP13 in osteoarthritic cartilage and its activity against type II collagen indicates that the enzyme plays a significant role in cartilage collagen degradation and must, therefore, form part of a complex target for proposed therapeutic interventions based on collagenase inhibition. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
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