Human MMP-8 ELISA Kit
KOA0272
1 Kit
ELISA
Human
156 pg/ml - 10000 pg/ml
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Human MMP-8 AccuSignal ELISA Kit - KOA0272
BB138268, CLG 1, CLG1, Collagenase 1, Collagenase 1 neutrophil, HNC, Matrix metallopeptidase 8, Matrix metalloproteinase 8 (neutrophil collagenase), Matrix metalloproteinase-8, MMP 8, MMP-8, Mmp8, MMP8_HUMAN, Neutrophil collagenase, PMNL CL, PMNL collagenase, PMNL-CL
ELISA Kit
156 pg/ml - 10000 pg/ml
Target Details
MMP8 - View All MMP8 Products
Human
Expression system for standard: E.coli; Immunogen sequence: F21-G467
Natural and recombinant human total MMP-8. There is no detectable cross-reactivity with other relevant proteins.
P22894 - UniProtKB
NP_001291370.1 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human MMP-8 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma (heparin) or saliva to each empty well. It is recommended that each human MMP-8 standard solution and each sample be measured in duplicate.
Formulation
Heparin Sodium
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Matrix metalloproteinase 8(MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic cross reactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase(HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation. The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.
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