Human MMP-12 ELISA Kit
KOA0581
1 Kit
ELISA
Human
62.5 pg/ml - 4000 pg/ml
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Human MMP-12 AccuSignal ELISA Kit - KOA0581
EC 3.4.24.65, HME, Macrophage elastase, Macrophage metalloelastase, Macrophage metaloelastase, Matrix metallopeptidase 12 (macrophage elastase), Matrix metalloprotease 12, Matrix metalloproteinase-12, MGC138506, MME, MMP 12, MMP-12, Mmp12, MMP12_HUMAN
ELISA Kit
62.5 pg/ml - 4000 pg/ml
Target Details
MMP12 - View All MMP12 Products
Human
Expression system for standard: NSO; Immunogen sequence: L17-C470
Natural and recombinant human MMP-12. There is no detectable cross-reactivity with other relevant proteins.
P39900 - UniProtKB
NP_002417.2 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human MMP-12 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each human MMP-12 standard solution and each sample be measured in duplicate.
Formulation
Heparin Sodium
1 Kit
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Matrix metalloproteinase-12(MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may involved in tissue injury and remodeling.
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