Human MMP-1 ELISA Kit
KOA0266
1 Kit
ELISA
Human
156 pg/ml - 10000 pg/ml
Shipping info:
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Product Details
Human MMP-1 AccuSignal ELISA Kit - KOA0266
27 kDa interstitial collagenase, CLG, CLGN, Fibroblast collagenase, Interstitial collagenase, Matrix metallopeptidase 1 (interstitial collagenase), Matrix Metalloproteinase 1, Matrix metalloproteinase-1, MMP 1, MMP-1, MMP1, MMP1_HUMAN
ELISA Kit
156 pg/ml - 10000 pg/ml
Target Details
MMP1 - View All MMP1 Products
Human
Expression system for standard: NSO; Immunogen sequence: F20-N469
Natural and recombinant human total MMP-1. There is no detectable cross-reactivity with other relevant proteins.
B4DN15 - UniProtKB
NP_001139410.1 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human MMP-1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma (heparin) to each empty well. It is recommended that each human MMP-1 standard solution and each sample be measured in duplicate.
Formulation
Heparin Sodium
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Matrix metalloproteinase-1(MMP-1) is also known as collagenase. Matrix metalloproteinases(MMPs) are zinc-dependent proteases that degrade extrMMP-1llular matrix proteins. The MMPs comprise a family of at least 20 proteolytic enzymes that play an essential role in tissue remodeling. Matrix metalloproteinase-1(MMP-1) plays an important role in the degradation of collagen in inflammatory diseases. MMP1(interstitial collagenase), MMP9(gelatinase B) and MMP12(macrophage elastase) are thought to be important in the development of emphysema. Smoking-induced MMP-1 might be important in the skin-ageing effects of tobacco smoking. The standard product used in this kit is human MMP-1 with the molecular mass of 51-53KDa. The detected MMP-1 includes zymogen and active enzyme.
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