Human LAP (TGF beta1) ELISA Kit
KOA0711
1 Kit
ELISA
Human
62.5 pg/ml - 4000 pg/ml
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Human LAP (TGF beta1) AccuSignal ELISA Kit - KOA0711
Camurati-Engelmann disease, CED, DPD1, LAP, Latency-associated peptide, TGF beta 1 protein, TGF-beta-1, TGFB, tgfb1, TGFB1_HUMAN, TGFbeta, Transforming growth factor beta 1
ELISA Kit
62.5 pg/ml - 4000 pg/ml
Target Details
TGFB1 - View All TGFB1 Products
Human
Expression system for standard: sf21; Immunogen sequence: L30-R278
Natural and recombinant human LAP(TGF-beta1). There is no detectable cross-reactivity with other relevant proteins.
P01137 - UniProtKB
NP_000651.3 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human LAP(TGF-beta1) standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human LAP(TGF-beta1) standard solution and each sample be measured in duplicate.
Formulation
Heparin Sodium
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide(beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. Processing and cleavage of the precursor protein between amino acids 278 and 279 results in the formation of LAP dimers and TGF beta dimers that then non-covalently associate with each other to form the small latent TGF beta complex. LAP is secreted and can be found in the extracellular matrix. In addition, LAP can also be expressed on platelets and activated regulatory T cells.
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