Human Kallistatin - Serpina4 ELISA Kit
KOA0502
1 Kit
ELISA
Human
156 pg/ml - 10000 pg/ml
Shipping info:
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Product Details
Human Kallistatin/Serpina4 AccuSignal ELISA Kit - KOA0502
KAIN_HUMAN, Kallikrein inhibitor, Kallistatin, Peptidase inhibitor 4, PI-4, PI4, Serpin A4, SERPINA4
ELISA Kit
156 pg/ml - 10000 pg/ml
Target Details
SERPINA4 - View All SERPINA4 Products
Human
Expression system for standard: NSO; Immunogen sequence: Q21-P427
Natural and recombinant human Kallistatin. There is no detectable cross-reactivity with other relevant proteins.
P10721 - UniProtKB
NP_000213.1 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human Kallistatin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Kallistatin standard solution and each sample be measured in duplicate.
Formulation
Heparin Sodium
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Serpin peptidase inhibitor, clade A(alpha-1 antiproteinase, antitrypsin), member 4, also called Kallistatin, is a protein that in humans is encoded by the SERPINA4 gene. This gene is mapped to chromosome 14q32.13. Kallistatin is a serine proteinase inhibitor(serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function. It shows that kallistatin plays a role in neointima hyperplasia. The potential role of kallistatin was investigated in angiogenesis in vitro and in vivo, and presented results demonstrating a novel role of the protein in the inhibition of angiogenesis and tumor growth.
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