Human Furin ELISA Kit
KOA0553
1 Kit
ELISA
Human
312 pg/ml - 20000 pg/ml
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Human Furin AccuSignal ELISA Kit - KOA0553
Dibasic processing enzyme, Dibasic-processing enzyme, FES upstream region, FUR, FURIN, Furin membrane associated receptor protein, FURIN_HUMAN, PACE, Paired basic amino acid residue cleaving enzyme, Paired basic amino acid residue-cleaving enzyme, PCSK3, Proprotein convertase subtilisin/kexin type 3, SPC1
ELISA Kit
312 pg/ml - 20000 pg/ml
Target Details
FURIN - View All FURIN Products
Human
Expression system for standard: NSO; Immunogen sequence: D108-E715
Natural and recombinant human Furin. There is no detectable cross-reactivity with other relevant proteins.
P09958 - UniProtKB
NP_001276752.1 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml human Furin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, tissue homogenates or cell lysates to each empty well. It is recommended that each human Furin standard solution and each sample be measured in duplicate.
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Furin (Fur), also known as PACE, is a protein that in humans is encoded by the FURIN gene. The protein encoded by this gene is an enzyme which belongs to the subtilisin-like proprotein convertase family. The FUR gene is located approximately 1 kb upstream of the FES gene on chromosome 15q25-q26. This gene is thought to play a role in tumor progression. Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 and gp41 prior to viral assembly. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Expression of furin in T-cells is required for maintenance of peripheral immune tolerance.
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