Human DKK-3 ELISA Kit
KOA0794
1 Kit
ELISA
Human
156 pg/ml - 10,000 pg/ml
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Human DKK-3 AccuSignal ELISA Kit - KOA0794
AW061014, C87148, Dickkopf (Xenopus laevis) homolog 3, Dickkopf 3, Dickkopf homolog 3 (Xenopus laevis), Dickkopf related protein 3, HDkk 3, Regulated in glioma, REIC, RIG, RIG like 5 6, RIG like 7 1
ELISA Kit
156 pg/ml - 10,000 pg/ml
Target Details
DKK3 - View All DKK3 Products
Human
Expression system for standard: NSO; Immunogen sequence: A22-I350
Natural and recombinant human DKK-3. There is no detectable cross-reactivity with other relevant proteins.
Q9UBP4 - UniProtKB
NP_001018067.1 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 10,000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human DKK-3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human DKK-3 standard solution and each sample be measured in duplicate.
Formulation
Heparin Sodium
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Dickkopf-related protein 3 is a protein that in humans is encoded by the DKK3 gene. This gene encodes a protein that is a member of the dickkopf family. It is mapped to 11p15.3. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Members of the Dkk-related family display unique patterns of mRNA expression in human and mouse tissues, and are secreted when expressed in 293T cells. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
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