Product Details
Human CD56/NCAM-1 AccuSignal ELISA Kit - KOA0409
CD56, N-CAM-1, NCAM-1, NCAM1, NCAM1_HUMAN, Neural cell adhesion molecule 1
ELISA Kit
156 pg/ml - 10000 pg/ml
Target Details
NCAM1 - View All NCAM1 Products
Human
Expression system for standard: NSO; Immunogen sequence: L20-G718
Natural and recombinant human CD56. There is no detectable cross-reactivity with other relevant proteins.
P13591 - UniProtKB
NP_000606.3 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human CD56 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human CD56 standard solution and each sample is measured in duplicate.
Formulation
Heparin Sodium
1 Kit
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin(Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23. This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.
Jennings MJ et al. (2022). NCAM1 and GDF15 are biomarkers of Charcot-Marie-Tooth disease in patients and mice. Brain.
Applications
EIA, ELISA
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