Datasheet Protocol

Human Caspase 3 ELISA Kit

Name: Human Caspase 3 ELISA Kit
Brand: Rockland Immunochemicals
Price: 589.0 USD
Availability: OutOfStock

Item No. KOA0860

Size: 1 Kit

Applications: ELISA

Reactivity: Human

Range: 31.2 pg/ml - 2000 pg/ml

$589.00 /Per Item

Out of stock

Ships in approximately 21 days

Add To Cart

Add to List

Bulk Orders

Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Specifications for Human Caspase 3 ELISA Kit

Product Details

Description: Human Caspase 3 AccuSignal ELISA Kit - KOA0860

Synonyms: CASP-3, CASP3, CASP 3, Caspase-3, Caspase3, Caspase 3, SCA-1, SCA 1, SCA1

Detection Kit Type: ELISA Kit

Detection Range: 31.2 pg/ml - 2000 pg/ml

Target Details

Gene Name: CASP3 - View All CASP3 Products

Reactivity: Human

Immunogen: Expression system for standard: E.coli; Immunogen sequence: S29-D175&A183-H277

Purity/Specificity: Natural and recombinant human Caspase 3. There is no detectable cross-reactivity with other relevant proteins.

Database Links:

P42574 - UniProtKB

NP_004337.2 - NCBI Protein

Application Details

Tested Applications: ELISA

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human Caspase 3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, tissue homogenates or serum to each empty well. It is recommended that each human Caspase 3 standard solution and each sample be measured in duplicate.

Formulation

Concentration: 1 Kit

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiration: See kit insert for complete instructions.

Background

Caspase-3 is a caspase protein that is encoded by the CASP3 gene. This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Related Protocols

ELISA Protocol

Sandwich ELISA Protocol for Collagen

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.