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WM46 Non-Viable Cell Pellet
WM46-03-0005
5 million cells
Frozen Cell Pellet
Human
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
WM46 Non-Viable Cell Pellet - WM46-03-0005
Melanoma, patient derived tumor, tumor models, skin cancer, xenograft
Human
Target Details
Cells were grown sub-confluent to approximately (2x10^6) cells in Tumor specialized media and then trypsinized. Cells were harvested and cell pellets were flash frozen and stored at -80°C.
Application Details
Frozen non-viable cell pellets can be used for in vitro experiments such as Western blotting and other immunoassays, genomic DNA isolation, STR profiling, and RNA isolation.
Cell Line Data
Human Melanoma
Non-Viable Pellets
V600E
R24C
WT
Hemizygous Deletion
Formulation
None
None
None
Shipping & Handling
Dry Ice
Store vial at -70° C. For extended storage, aliquot contents to minimize freeze/thaw cycles.
Expiration date is one (1) year from date of receipt.
Non-viable cell pellet was generated from cell line WM46. WM46 is a metastatic human melanoma cell line that displays a pigmented fibroblastic morphology. This cell line was established from left chest & abdomen in a 43-year-old female patient with level IV superficial spreading melanoma and a tumor thickness of 1.44. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line expresses PTEN loss of function including hemizygous deletion, and R24C mutation at position 24 in the CDK4 gene. The R24C mutation results in an amino acid substitution at codon 24 in CDK4, from an arginine (R) to cysteine (C). This mutation abolishes the ability of CDK4 to bind to p16(INK4a). WM46 cells produce xenograft tumors when injected into immunocompromised mice.