Datasheet is currently unavailable. Try again or CONTACT US
WM1727A Non-Viable Cell Pellet
WM1727A-03-0005
5 million cells
Frozen Cell Pellet
Human
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
WM1727A Non-Viable Cell Pellet - WM1727A-03-0005
Melanoma, patient derived tumor, tumor models, skin cancer, xenograft
Human
Target Details
Cells were grown sub-confluent to approximately (2x10^6) cells in Tumor specialized media and then trypsinized. Cells were harvested and cell pellets were flash frozen and stored at -80°C.
Application Details
Frozen non-viable cell pellets can be used for in vitro experiments such as Western blotting and other immunoassays, genomic DNA isolation, STR profiling, and RNA isolation.
Cell Line Data
Human Melanoma
Non-Viable Pellets
V600E
WT
WT
Hemizygous Deletion
Formulation
None
None
None
Shipping & Handling
Dry Ice
Store vial at -70° C. For extended storage, aliquot contents to minimize freeze/thaw cycles.
Expiration date is one (1) year from date of receipt.
Non-viable cell pellet was generated from cell line WM1727. WM1727A is a metastatic human melanoma cell line that displays pigmented melanocytic morphology and float easily in culture medium. WM1727A cells produce xenograft tumors when injected into immunocompromised mice. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line also expresses PTEN loss of function including hemizygous deletion and is wild type for N-RAS, c-KIT, and CDK4.